我们组最新发表的JACS进入Spotlights on Recent JACS Publications推荐
2020年05月05日
https://pubs.acs.org/doi/full/10.1021/jacs.0c04305
Oxidative stress and abnormal energy metabolism are hallmarks of several neurological disorders, including Parkinson’s disease and Alzheimer’s disease. Measuring the levels of reactive oxygen species and energy sources in the cell enables a better understanding of how oxidative and energetic processes contribute to the progression of these diseases, which may facilitate the development of more effective treatments. However, current methods to detect such analytes require extensive sample preparation and are therefore unable to provide real-time monitoring of concentration changes in response to specific stimuli.
Now, Yang Tian and co-workers have developed a fluorescent probe that can selectively and simultaneously detect mitochondrial H
2O
2 and ATP to characterize the interplay between oxidative stress and energy metabolism under different conditions (DOI:
10.1021/jacs.0c00771). Using this probe, the researchers measured H
2O
2 and ATP levels in neurons that had been exposed to various reactive oxygen species and discovered exposure time dependence in the permanence of the observed fluctuations. They then used the probe to evaluate zebrafish in an oxygen-deprived environment and found that the fish exhibited increased H
2O
2 production and decreased ATP synthesis, indicating oxidative damage and energy deficit. This work helps to uncover the role of H
2O
2 and ATP in neurodegeneration and provides a general fluorescence-based strategy for multiple molecule imaging.
